Step by step:
Connect GND and USB UART RX to MCU TX and vice versa. Start check or
program in STC-ISP or stcgal, which will then wait for the MCU. Only
then apply power to the MCU's VCC.
In my experience, STC-ISP can be a bit finicky with PL2303 USB UARTs.
stcgal works quite a bit more reliable with those.
Regarding your other questions:
> I managed mcu check with stc isp but only occasionally. Sometimes I can
not communicate with the MCU. But I do not know why.
Good, that probably means you have the connection between UART and MCU
working fine. Try to lower the baudrate, and make sure to always toggle
power when you want to initiate the bootloader.
> Do you know where to find and how to flash bootloader?
The bootloader is preprogrammed in the factory. It cannot be
overwritten. Well, it is not supposed to be, anyway. Maybe there are
undocumented ways to do it, who knows.
> and what are the
two pins that disable the bootloader to see if this is not the case on
my pcb.
Not sure, should be either P1.0/P1.1 or P3.2/P3.3. But check out the
list of device options in STC-ISP for your part, it is documented there.